Selected article for: "PCR polymerase chain reaction and stop codon"
Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis
  • Document date: 2010_1_7
    • ID: 1mzq227n_8
    Snippet: Cloning and expression of P gene of CHPV in pAcGFP1N1 P gene of CHPV was amplified by reverse transcriptase -polymerase chain reaction (RT-PCR) using PFloF1 (59-TCTCGAGCTCAAGCTCCACCATGGAAGACTCTCAACT-AT-39) and PGFPR1 (59-CCGCGGTACCGTCGAATTGAAC-TGGGGTTCAAG-39) primers without stop codon and cloned into pAcGFP1N1 vector (named pAcGFP1N1-CHPV-P). HEK-293 cells were transfected with 2 mg each of pAcGFP1N1 and pAcGFP1N1-CHPV-P. Transfected cells were .....
    Document: Cloning and expression of P gene of CHPV in pAcGFP1N1 P gene of CHPV was amplified by reverse transcriptase -polymerase chain reaction (RT-PCR) using PFloF1 (59-TCTCGAGCTCAAGCTCCACCATGGAAGACTCTCAACT-AT-39) and PGFPR1 (59-CCGCGGTACCGTCGAATTGAAC-TGGGGTTCAAG-39) primers without stop codon and cloned into pAcGFP1N1 vector (named pAcGFP1N1-CHPV-P). HEK-293 cells were transfected with 2 mg each of pAcGFP1N1 and pAcGFP1N1-CHPV-P. Transfected cells were observed under florescent microscope periodically to check the expression of green fluorescent protein (GFP) fused CHPV P protein and confirmed by western blotting using anti-GFP antibodies (Invitrogen, Carlsbad, CA, USA).

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