Selected article for: "Qiagen RNeasy kit and specific primer"
Author: Suthar, Mehul S.; Ma, Daphne Y.; Thomas, Sunil; Lund, Jennifer M.; Zhang, Nu; Daffis, Stephane; Rudensky, Alexander Y.; Bevan, Michael J.; Clark, Edward A.; Kaja, Murali-Krishna; Diamond, Michael S.; Gale, Michael
Title: IPS-1 Is Essential for the Control of West Nile Virus Infection and Immunity
  • Document date: 2010_2_5
    • ID: 094d0rn6_45
    Snippet: For analysis of viremia, serum was separated (BD Microtainer tube SST) and RNA was extracted as previously described [8] . WNV RNA copy number was measured by RT-quantitative PCR (RT-qPCR) as previously described [63] . For cultured cells, total RNA was extracted using the RNeasy kit (Qiagen), DNase treated (Ambion) and evaluated for ISG49, ISG56, IFN-b, RIG-I, and MDA5 RNA expression by one-step SYBR Green RT-qPCR. Specific primer sets for ISG-4.....
    Document: For analysis of viremia, serum was separated (BD Microtainer tube SST) and RNA was extracted as previously described [8] . WNV RNA copy number was measured by RT-quantitative PCR (RT-qPCR) as previously described [63] . For cultured cells, total RNA was extracted using the RNeasy kit (Qiagen), DNase treated (Ambion) and evaluated for ISG49, ISG56, IFN-b, RIG-I, and MDA5 RNA expression by one-step SYBR Green RT-qPCR. Specific primer sets for ISG-49, ISG-56, RIG-I, and IFN-b have been described previously [30, 64] . Primer sets for MDA5 are: 59-GTGGTCGAGCCAGAGCTGAT and 39-TGTCTCATG-TTCGATAACTCCTGAA.

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