Author: Paesen, Guido C.; Collet, Axelle; Sallamand, Corinne; Debart, Françoise; Vasseur, Jean-Jacques; Canard, Bruno; Decroly, Etienne; Grimes, Jonathan M.
Title: X-ray structure and activities of an essential Mononegavirales L-protein domain Document date: 2015_11_9
ID: 1taxqkk2_26
Snippet: Methylation of GTP was determined in the same buffer (minus the 3 H-SAM and RNA), using the EPIgeneous methyltransferase assay kit (Cisbio), which measures the generation of SAH, as it competes with d2-coupled SAH for binding to a Lumi4-Tb-labelled anti-SAH antibody, affecting the TR-FRET signal between these compounds. In practice, MTase reactions were stopped after 16 h by addition of the detection reagents, and 1 h later the TR-FRET signal was.....
Document: Methylation of GTP was determined in the same buffer (minus the 3 H-SAM and RNA), using the EPIgeneous methyltransferase assay kit (Cisbio), which measures the generation of SAH, as it competes with d2-coupled SAH for binding to a Lumi4-Tb-labelled anti-SAH antibody, affecting the TR-FRET signal between these compounds. In practice, MTase reactions were stopped after 16 h by addition of the detection reagents, and 1 h later the TR-FRET signal was monitored using a PHERAstar Flashlamp plate reader.
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