Selected article for: "equal volume and formamide EDTA gel loading buffer"
Author: Paesen, Guido C.; Collet, Axelle; Sallamand, Corinne; Debart, Françoise; Vasseur, Jean-Jacques; Canard, Bruno; Decroly, Etienne; Grimes, Jonathan M.
Title: X-ray structure and activities of an essential Mononegavirales L-protein domain
  • Document date: 2015_11_9
    • ID: 1taxqkk2_29
    Snippet: NTPase assay. Samples combining CR-VI þ (4 mM) with 0.5 mCi [a-32 P]-GTP or 0.5 mCi [a-32 P]-ATP in 40 mM Tris-HCl (pH 8.5), 1 mM DTT and 5 mM MgCl 2 were incubated at 37°C. Reactions were stopped by adding an equal volume of formamide/EDTA gel-loading buffer, and hydrolysis products were separated over a 20% polyacrylamide/8 M urea gel before phosphor-imaging......
    Document: NTPase assay. Samples combining CR-VI þ (4 mM) with 0.5 mCi [a-32 P]-GTP or 0.5 mCi [a-32 P]-ATP in 40 mM Tris-HCl (pH 8.5), 1 mM DTT and 5 mM MgCl 2 were incubated at 37°C. Reactions were stopped by adding an equal volume of formamide/EDTA gel-loading buffer, and hydrolysis products were separated over a 20% polyacrylamide/8 M urea gel before phosphor-imaging.

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