Author: Takhampunya, Ratree; Korkusol, Achareeya; Pongpichit, Chalermpol; Yodin, Komsan; Rungrojn, Artharee; Chanarat, Nitima; Promsathaporn, Sommai; Monkanna, Taweesak; Thaloengsok, Sasikanya; Tippayachai, Bousaraporn; Kumfao, Naruemon; Richards, Allen L.; Davidson, Silas A.
Title: Metagenomic Approach to Characterizing Disease Epidemiology in a Disease-Endemic Environment in Northern Thailand Document date: 2019_2_26
ID: 0gi6qzw0_63_1
Snippet: ost often rely upon. However, there is no standardized protocol for all laboratories and contamination from reagents and the environment can complicate the analysis. Therefore, conventional methods were also employed as confirmatory assays in this study. The 16S sequence can only discriminate pathogens to the genus level with confidence. However, one genus may consist of multiple species, some of which may not be pathogenic to humans or animals. .....
Document: ost often rely upon. However, there is no standardized protocol for all laboratories and contamination from reagents and the environment can complicate the analysis. Therefore, conventional methods were also employed as confirmatory assays in this study. The 16S sequence can only discriminate pathogens to the genus level with confidence. However, one genus may consist of multiple species, some of which may not be pathogenic to humans or animals. Therefore, it is necessary to further identify the bacteria genera detected by NGS to the species level using conventional PCR or Sanger sequencing.
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