Author: Allemandou, Aude; Grasland, Béatrice; Hernandez-Nignol, Anne-Cécile; Kéranflec'h, André; Cariolet, Roland; Jestin, André
Title: Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein Document date: 2011_3_24
ID: 1fl0we2a_65
Snippet: PCV-2 genomic loads found in the organs of the PCV-2a/motif 2b transfected piglets were not remnants of plasmid input. Indeed, a previous study in our laboratory showed that after injection by intra-muscular route of plasmid DNA in SPF pigs, that the plasmid DNA was detected until 28 dpt in the injected muscle and 17 dpt in the draining pre-scapular lymph nodes. In other organs such as the liver, the spleen and the lungs, injected plasmid DNA was.....
Document: PCV-2 genomic loads found in the organs of the PCV-2a/motif 2b transfected piglets were not remnants of plasmid input. Indeed, a previous study in our laboratory showed that after injection by intra-muscular route of plasmid DNA in SPF pigs, that the plasmid DNA was detected until 28 dpt in the injected muscle and 17 dpt in the draining pre-scapular lymph nodes. In other organs such as the liver, the spleen and the lungs, injected plasmid DNA was present until 1 dpt in few quantity [33] . In our study, piglets were euthanized after 35 dpt, later than the last detection of plasmids in the previous experiment. These results confirmed that the PCV-2 genomes detected in organs were viral DNA and not input plasmid DNA.
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