Author: Gardner, Shea N.; Jaing, Crystal J.; Elsheikh, Maher M.; Peña, José; Hysom, David A.; Borucki, Monica K.
Title: Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes Document date: 2014_8_3
ID: 1e44usl6_7
Snippet: Requiring that primers fall within 0.5 bases of the ends of each region facilitates the creation of amplicons which should overlap across a genome, allowing full genome assembly from the amplified products. There may not be amplicons covering the extreme 5 and 3 ends of a target sequence, since the first and last primers may be located some distance (maximum of /2) from the ends. Rapid Amplification of cDNA Ends (RACE) PCR would be necessary to a.....
Document: Requiring that primers fall within 0.5 bases of the ends of each region facilitates the creation of amplicons which should overlap across a genome, allowing full genome assembly from the amplified products. There may not be amplicons covering the extreme 5 and 3 ends of a target sequence, since the first and last primers may be located some distance (maximum of /2) from the ends. Rapid Amplification of cDNA Ends (RACE) PCR would be necessary to amplify the genome ends not covered by an overlapping region, priming with the reverse complement of the run tiled primers primers closest to the end so as to prime toward the edge of the genome.
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