Author: Hedegaard, Chris J.; Strube, Mikael L.; Hansen, Marie B.; Lindved, Bodil K.; Lihme, Allan; Boye, Mette; Heegaard, Peter M. H.
Title: Natural Pig Plasma Immunoglobulins Have Anti-Bacterial Effects: Potential for Use as Feed Supplement for Treatment of Intestinal Infections in Pigs Document date: 2016_1_29
ID: 1tcpaigw_16
Snippet: Indirect ELISA for determination of relative avidity of IgG: After coating the wells with E. coli O138 DOC extract in 2-fold serial dilutions starting at 66.6 μg/ml, plates were blocked and subsequently washed, followed by incubation with 6 mg/ml ppIgG (in 2.5% casein pH 7) for 1 hour. Following the wash steps, 100 μl of PBS-T with 0, 2, 4 or 6 M urea was added to designated wells, and after 30 minutes of incubation and shaking wells were washe.....
Document: Indirect ELISA for determination of relative avidity of IgG: After coating the wells with E. coli O138 DOC extract in 2-fold serial dilutions starting at 66.6 μg/ml, plates were blocked and subsequently washed, followed by incubation with 6 mg/ml ppIgG (in 2.5% casein pH 7) for 1 hour. Following the wash steps, 100 μl of PBS-T with 0, 2, 4 or 6 M urea was added to designated wells, and after 30 minutes of incubation and shaking wells were washed again, followed by incubation with HRP-conjugated rabbit anti-porcine Ig diluted 1/7000 for 1 hour. The ELI-SAs were developed and read as described above.
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