Author: Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian
Title: Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches Document date: 2018_8_16
ID: 184kmuiy_18
Snippet: After deparaffinization and rehydration, paraffin sections were put in citrate buffer (pH 6) at 90-95°C for 15 min to retrieve antigen. Then, the sections were put in 0.3% H 2 O 2 to quench endogenous peroxidase and washed in PBS. 5% bovine serum albumin were incubated on sections for 30 min to close the non-specific antibody binding sites. After blocking with 5% bovine serum albumin, sections were incubated with goat anti-pig IgA overnight at 4.....
Document: After deparaffinization and rehydration, paraffin sections were put in citrate buffer (pH 6) at 90-95°C for 15 min to retrieve antigen. Then, the sections were put in 0.3% H 2 O 2 to quench endogenous peroxidase and washed in PBS. 5% bovine serum albumin were incubated on sections for 30 min to close the non-specific antibody binding sites. After blocking with 5% bovine serum albumin, sections were incubated with goat anti-pig IgA overnight at 4°C. Biotinylated secondary antibodies were added to the sections for 1 h at room temperature, and treated with SABC for 60 min. Sections were counterstained with hematoxylin and images were obtained using a light microscope (BH-2; Olympus). Different fields of each tissue in each piglet were counted for the statistical analysis.
Search related documents:
Co phrase search for related documents- anti pig iga and bovine serum albumin: 1
- anti pig iga and goat anti pig iga: 1, 2
- bind site and bovine serum: 1
- bind site and bovine serum albumin: 1
- biotinylated secondary antibody and bovine serum: 1
- biotinylated secondary antibody and bovine serum albumin: 1
- biotinylated secondary antibody and citrate buffer: 1, 2
- biotinylated secondary antibody and endogenous peroxidase: 1
- biotinylated secondary antibody and endogenous peroxidase quench: 1
- bovine serum albumin and citrate buffer: 1
- bovine serum albumin and endogenous peroxidase: 1, 2
- bovine serum albumin and goat anti pig iga: 1
- bovine serum albumin and light microscope: 1
- bovine serum and citrate buffer: 1, 2
- bovine serum and endogenous peroxidase: 1, 2, 3
- bovine serum and goat anti pig iga: 1
- bovine serum and light microscope: 1
- citrate buffer and endogenous peroxidase: 1, 2, 3, 4, 5, 6
- citrate buffer and endogenous peroxidase quench: 1
Co phrase search for related documents, hyperlinks ordered by date