Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches

Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian

According to the cell lineage, DCs can be divided into two major subsets that include plasmacytoid DCs (pDCs) and myeloid DCs, with the latter commonly referred to as conventional DCs (cDCs) [5] . DCs involved in intestinal immunity were investigated by cDCs induced from bone marrow cells or pDCs induced from blood mononuclear cells so far [6, 7] . But neither pDCs nor cDCs represent the reality of DCs within PPs. Indeed it is difficult to obtain the DCs within PPs in human. Unlike the mouse (isolated PPs), pig had continuous PPs (aggregated PPs), just like human. It is possible to get DCs within PPs in pig. The pig would be an animal model for studying DCs within PPs in human. Therefore, it is important to establish a method that isolate DCs from porcine PPs.