Author: Vittucci, Anna Chiara; Spuri Vennarucci, Valentina; Grandin, Annalisa; Russo, Cristina; Lancella, Laura; Tozzi, Albero Eugenio; Bartuli, Andrea; Villani, Alberto
Title: Pertussis in infants: an underestimated disease Document date: 2016_8_15
ID: 10xqdm7m_12
Snippet: The bacterial DNA was processed immediately on Taqman platform (Applied Biosystem) with Bordetella R-geneâ„¢ assay (Argene, Biomerieux, Marcy l'Etoile, France), able to amplify a fragment of 191 bp of the target region IS481 for BP. The ready-to-use amplification mixture included primers, dNTPs, amplification buffer, Taq Polymerase, probes specific for Bordetella and for the IC. The target DNA was amplified through the Taq Polymerase activation a.....
Document: The bacterial DNA was processed immediately on Taqman platform (Applied Biosystem) with Bordetella R-gene™ assay (Argene, Biomerieux, Marcy l'Etoile, France), able to amplify a fragment of 191 bp of the target region IS481 for BP. The ready-to-use amplification mixture included primers, dNTPs, amplification buffer, Taq Polymerase, probes specific for Bordetella and for the IC. The target DNA was amplified through the Taq Polymerase activation at 95°C for 15 min, and 45 repeats of denaturation at 95°C for 10 s and hybridization/elongation at 60°C for 40 s.
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