Author: Vittucci, Anna Chiara; Spuri Vennarucci, Valentina; Grandin, Annalisa; Russo, Cristina; Lancella, Laura; Tozzi, Albero Eugenio; Bartuli, Andrea; Villani, Alberto
Title: Pertussis in infants: an underestimated disease Document date: 2016_8_15
ID: 10xqdm7m_39
Snippet: The IS481 sequence is represented from 50 up to 238 copies per cel in BP considering this an advantage in term of high sensitive detection. Notwithstanding, microbiologist, infectious disease practitioners and pediatrics should be acquainted with the low presence of this target in Bordetella holmesii (8 to 10 copies per genome) and Bordetella bronchiseptica as well [28] [29] [30] . Some laboratories perform a second PCR assay on IS481-positive sp.....
Document: The IS481 sequence is represented from 50 up to 238 copies per cel in BP considering this an advantage in term of high sensitive detection. Notwithstanding, microbiologist, infectious disease practitioners and pediatrics should be acquainted with the low presence of this target in Bordetella holmesii (8 to 10 copies per genome) and Bordetella bronchiseptica as well [28] [29] [30] . Some laboratories perform a second PCR assay on IS481-positive specimens using either a BP-or a Bordetella holmesii-specific target or both. However, these other targets are at least ten fold less sensitive than IS481 target since they are present in fewer copies of the genome. A number of potential target sequences have been proposed to increase the diagnosis of BP and Bordetella holmesii but there is no recommendation on what is the best PCR diagnosis strategy to use at the present time [16] .
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