Author: Joshua A. Hayward; Mary Tachedjian; Adam Johnson; Tamsin B. Gordon; Jie Cui; Glenn A. Marsh; Michelle L. Baker; Lin-Fa Wang; Gilda Tachedjian
Title: Bats Possess Unique Variants of the Antiviral Restriction Factor Tetherin Document date: 2020_4_9
ID: eksi9yia_17
Snippet: To verify the expression of bat tetherin isoform A, a cDNA library was prepared from P. alecto spleen tissue, comprising a number of immune cell types, and assessed for the presence of transcripts matching that identified in the P. alecto transcriptome. Primers were designed to flank the open reading frame of tetherin isoform A, and amplicons were generated by PCR. Amplicons were then cloned into plasmid vectors to allow for DNA sequencing. This .....
Document: To verify the expression of bat tetherin isoform A, a cDNA library was prepared from P. alecto spleen tissue, comprising a number of immune cell types, and assessed for the presence of transcripts matching that identified in the P. alecto transcriptome. Primers were designed to flank the open reading frame of tetherin isoform A, and amplicons were generated by PCR. Amplicons were then cloned into plasmid vectors to allow for DNA sequencing. This analysis revealed two additional isoforms of tetherin, isoforms B & C.
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