Selected article for: "luciferase activity and pathway signaling"
Author: Cao, Liyan; Ge, Xuying; Gao, Yu; Herrler, Georg; Ren, Yudong; Ren, Xiaofeng; Li, Guangxing
Title: Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-ß production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway
  • Document date: 2015_8_18
    • ID: 0qt7eth0_12
    Snippet: IPS-1 is an adapter molecule of RIG-I, the data showed that PEDV blocked IPS-1-induced IFN-β production in dsRNA signaling pathway, thus, we speculated that RIG-I-induced IFN-β production in this signaling pathway was also inhibited. To verify it, mock-and PEDVinfected IECs were transfected with RIG-I expression and IFN-β reporter plasmids. The results showed that IFN-β luciferase activity was markedly increased in IECs overexpressing RIG-I, .....
    Document: IPS-1 is an adapter molecule of RIG-I, the data showed that PEDV blocked IPS-1-induced IFN-β production in dsRNA signaling pathway, thus, we speculated that RIG-I-induced IFN-β production in this signaling pathway was also inhibited. To verify it, mock-and PEDVinfected IECs were transfected with RIG-I expression and IFN-β reporter plasmids. The results showed that IFN-β luciferase activity was markedly increased in IECs overexpressing RIG-I, but was completely inhibited by PEDV infection. And the IFN-β reporter signal could be observed in IECs stimulated with poly (I:C), while this signal could be sharply reduced in RIG-Itransfected, poly (I:C)-stimulated and PEDV-infected IECs (Fig. 4b) .

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