Author: Girsch, James H.; Walters, Katherine; Jackson, Wallen; Grose, Charles
Title: Progeny Varicella-Zoster Virus Capsids Exit the Nucleus but Never Undergo Secondary Envelopment during Autophagic Flux Inhibition by Bafilomycin A1 Document date: 2019_8_13
ID: 1qbklvqy_25
Snippet: Cell culture and virus. MRC5 fibroblasts (ATCC) and MeWo cells (Sloan Kettering Institute) were grown in minimum essential medium (MEM) (Life Technologies) supplemented with 7% fetal bovine serum, L-glutamine, nonessential amino acids, and penicillin-streptomycin. When the cells were ϳ90% confluent, they were inoculated with trypsin-dispersed infected cells at a ratio of one infected cell to eight uninfected cells (18) . The titer of the inoculu.....
Document: Cell culture and virus. MRC5 fibroblasts (ATCC) and MeWo cells (Sloan Kettering Institute) were grown in minimum essential medium (MEM) (Life Technologies) supplemented with 7% fetal bovine serum, L-glutamine, nonessential amino acids, and penicillin-streptomycin. When the cells were ϳ90% confluent, they were inoculated with trypsin-dispersed infected cells at a ratio of one infected cell to eight uninfected cells (18) . The titer of the inoculum was 1 million infectious foci/ml. The virus strain was the completely sequenced low-passage-number VZV-32 strain (50) .
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