Selected article for: "liquid scintillation fluid and scintillation fluid"
Author: Paesen, Guido C.; Collet, Axelle; Sallamand, Corinne; Debart, Françoise; Vasseur, Jean-Jacques; Canard, Bruno; Decroly, Etienne; Grimes, Jonathan M.
Title: X-ray structure and activities of an essential Mononegavirales L-protein domain
  • Document date: 2015_11_9
    • ID: 1taxqkk2_25
    Snippet: MTase activity assays. These were performed by combining 4 mM CR-VI þ with 0.7 mM of the purified and validated synthetic RNAs, 10 mM SAM and 0.33 mM 3 H-SAM (Perkin Elmer) in 40 mM Tris-HCl (pH 8.5) and 1 mM dithiothreitol (DTT). Reactions (at 30°C) were stopped by a 10-fold dilution in 100 mM ice-cold SAH and the samples were transferred to DEAE filtermats (Perkin Elmer) using a Filtermat Harvester (Packard Instruments). The RNA-retaining mat.....
    Document: MTase activity assays. These were performed by combining 4 mM CR-VI þ with 0.7 mM of the purified and validated synthetic RNAs, 10 mM SAM and 0.33 mM 3 H-SAM (Perkin Elmer) in 40 mM Tris-HCl (pH 8.5) and 1 mM dithiothreitol (DTT). Reactions (at 30°C) were stopped by a 10-fold dilution in 100 mM ice-cold SAH and the samples were transferred to DEAE filtermats (Perkin Elmer) using a Filtermat Harvester (Packard Instruments). The RNA-retaining mats were washed twice with 10 mM ammonium formate (pH 8.0), twice with water and once with ethanol. They were then soaked with liquid scintillation fluid, allowing the measurement of 3 H-methyl transfer to the RNA substrates using a Wallac MicroBeta TriLux Liquid Scintillation Counter 13 .

    Search related documents:
    Co phrase search for related documents
    • ammonium formate and pH 10 mm ammonium formate: 1
    • ammonium formate and scintillation fluid: 1
    • DEAE filtermat and Packard Instruments Filtermat Harvester: 1
    • DEAE filtermat and Perkin Elmer DEAE filtermat: 1
    • liquid scintillation fluid and pH 10 mm ammonium formate: 1
    • liquid scintillation fluid and scintillation fluid: 1
    • mtase activity and RNA substrate: 1, 2, 3, 4, 5, 6, 7
    • mtase activity and synthetic rna: 1, 2
    • Packard Instruments Filtermat Harvester and Perkin Elmer DEAE filtermat: 1
    • pH 10 mm ammonium formate and scintillation fluid: 1
    • RNA retain and synthetic rna: 1
    • RNA substrate and synthetic rna: 1, 2, 3, 4, 5, 6, 7, 8, 9