Selected article for: "cell stock and frozen cell stock"

Author: Moss, Ronald B
Title: Prospects for control of emerging infectious diseases with plasmid DNA vaccines
  • Document date: 2009_9_7
  • ID: 1a5u7uux_11
    Snippet: Production of DNA vaccines starts with E. coli cells which are transformed with the plasmid of interest. These cells are grown and stored frozen in a stock of vials called a Master Cell Bank. Growth of the E. coli is typically done via a fermentation process similar to that used in the manufacturing of certain alcoholic beverages. The recovery process then requires lysis of the cells, in order to release the plasmid retained within the E. coli ce.....
    Document: Production of DNA vaccines starts with E. coli cells which are transformed with the plasmid of interest. These cells are grown and stored frozen in a stock of vials called a Master Cell Bank. Growth of the E. coli is typically done via a fermentation process similar to that used in the manufacturing of certain alcoholic beverages. The recovery process then requires lysis of the cells, in order to release the plasmid retained within the E. coli cells. DNA is then purified using various chromatographic methods.

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