Author: Wang, Li; Xia, Tian; Guo, Tiantian; Ru, Yi; Jiang, Yanping; Cui, Wen; Zhou, Han; Qiao, Xinyuan; Tang, Lijie; Xu, Yigang; Li, Yijing
Title: Recombinant Lactobacillus casei Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits Document date: 2019_11_2
ID: 1apf9w7j_20
Snippet: The first group was injected with a single does of a commercial inactivated RHDV vaccine. The second group was orally administered 5 mL L. casei pPG-eGFP-VP60/LC393 (1 × 10 10 CFU/mL). The third group, serving as unimmunized control, was inoculated with 5 mL of orally administered PBS. The rabbits were immunized orally once a day for three consecutive days and boosted twice at 2-week intervals ( Figure 2 ). Serum, nasal washes, vaginal lotions, .....
Document: The first group was injected with a single does of a commercial inactivated RHDV vaccine. The second group was orally administered 5 mL L. casei pPG-eGFP-VP60/LC393 (1 × 10 10 CFU/mL). The third group, serving as unimmunized control, was inoculated with 5 mL of orally administered PBS. The rabbits were immunized orally once a day for three consecutive days and boosted twice at 2-week intervals ( Figure 2 ). Serum, nasal washes, vaginal lotions, tears, and fecal samples were obtained on days 0, 7, 14, 21, 28, and 35 after immunization, and were stored at −20 • C until use. Fecal samples were pretreated according to a previously published method [35] .
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