Recombinant Lactobacillus casei Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits

Wang, Li; Xia, Tian; Guo, Tiantian; Ru, Yi; Jiang, Yanping; Cui, Wen; Zhou, Han; Qiao, Xinyuan; Tang, Lijie; Xu, Yigang; Li, Yijing

To assess immunoprotective efficacy, the three groups of rabbits administered pPG-eGFP-VP60/LC393, inactivated vaccine, or PBS were infected with 1 mL RHDV (RNA copy number of 1.0 × 10 5.97 ) at 7 days after the last booster. Our results show that all rabbits in the PBS group died within three days post-challenge, while the rabbits immunized with recombinant L. casei or inactivated vaccine were completely protected against challenge with RHDV ( Figure 7 ). , nasal cavity (c), and tears (d) was performed by ELISA using RHDV as the coating antigen. The samples from each group (6 rabbits) were pooled and then analyzed as one sample in triplicate. Bars represent the mean ± standard deviation (SD) in each group. Different letters (a vs. b, a vs. c, b vs. c) indicate significant differences (p < 0.01) at the same time point.