Selected article for: "PCR amplification and previous study"
Author: Wang, Li; Xia, Tian; Guo, Tiantian; Ru, Yi; Jiang, Yanping; Cui, Wen; Zhou, Han; Qiao, Xinyuan; Tang, Lijie; Xu, Yigang; Li, Yijing
Title: Recombinant Lactobacillus casei Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits
  • Document date: 2019_11_2
    • ID: 1apf9w7j_8
    Snippet: The sequences of the primers used for PCR amplification of VP60 were as follows: 5 -ATGGAGGGCAAAACCCGCACAGCGC-3 ; 5 -TCAGACATAAGAAAAGCCATTGGCT-3 . pPG-T7g10-PPT, constructed in our previous study [33] , was used as a constitutive expression plasmid in this study; it encoded resistance to chloramphenicol (Cm) and contained an HCE promoter, T7g10 enhancer, PgsA anchor, and the rrnBT1T2 terminator. Plasmids pMD18-T simple-eGFP and pMD18-T simple-VP6.....
    Document: The sequences of the primers used for PCR amplification of VP60 were as follows: 5 -ATGGAGGGCAAAACCCGCACAGCGC-3 ; 5 -TCAGACATAAGAAAAGCCATTGGCT-3 . pPG-T7g10-PPT, constructed in our previous study [33] , was used as a constitutive expression plasmid in this study; it encoded resistance to chloramphenicol (Cm) and contained an HCE promoter, T7g10 enhancer, PgsA anchor, and the rrnBT1T2 terminator. Plasmids pMD18-T simple-eGFP and pMD18-T simple-VP60 were constructed in our laboratory previously.

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