Author: Schwarz, Megan C.; Sourisseau, Marion; Espino, Michael M.; Gray, Essanna S.; Chambers, Matthew T.; Tortorella, Domenico; Evans, Matthew J.
Title: Rescue of the 1947 Zika Virus Prototype Strain with a Cytomegalovirus Promoter-Driven cDNA Clone Document date: 2016_9_28
ID: 0i1abjfa_17
Snippet: To further compare the growth properties of the rescued and the parent viruses, multicycle growth curve experiments were performed in which Vero cells were infected (MOI of 0.01) for 1 h with either the rescued wild-type virus or the parental virus, followed by several washes to remove input virus. Supernatants were collected and filtered daily up to 4 days postinfection, and then titers of the supernatants were determined by Vero cell TCID 50 as.....
Document: To further compare the growth properties of the rescued and the parent viruses, multicycle growth curve experiments were performed in which Vero cells were infected (MOI of 0.01) for 1 h with either the rescued wild-type virus or the parental virus, followed by several washes to remove input virus. Supernatants were collected and filtered daily up to 4 days postinfection, and then titers of the supernatants were determined by Vero cell TCID 50 assay. The results of the growth curve revealed that our rescued virus has growth kinetics comparable to those of the parental virus (Fig. 4F) .
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