RNA disruption is associated with response to multiple classes of chemotherapy drugs in tumor cell lines

Narendrula, Rashmi; Mispel-Beyer, Kyle; Guo, Baoqing; Parissenti, Amadeo M.; Pritzker, Laura B.; Pritzker, Ken; Masilamani, Twinkle; Wang, Xiaohui; Lannér, Carita

The A2780 parental cell line was acquired from the European Collection of Cell Cultures. The development and characterization of the docetaxel-resistant A2780DXL and carboplatin-resistant A2780CBN cell lines used in this study were described previously [23] . Cell lines were maintained in RPMI-1640 culture medium containing 10 % FBS, 1 % penicillin (10,000 units/ml), and 1 % streptomycin (10,000 μg/ml) (Hyclone Laboratories, Logan, UT, USA). Docetaxel resistance in the A2780DXL cell line was maintained by treating the cells with 0.4 μM docetaxel in complete medium weekly. Carboplatin resistance in A2780CBN cells was similarly maintained by treating the cells with 22 μM carboplatin. The CaOV3 and MCF-7 cell lines, a gift from Dr. Linda Malkas, were maintained in DMEM containing 10 % FBS, 1 % penicillin (10,000 units/ml), and 1 % streptomycin (10,000 μg/ml) (Hyclone Laboratories, Logan, UT, USA). The SKBR3 and BT474 cell lines, a gift from Dr. Robert Lafrenie, were also maintained in DMEM containing 10 % FBS, 1 % penicillin (10,000 units/ml), and 1 % streptomycin (10,000 μg/ml) (Hyclone Laboratories, Logan, UT, USA). The MDA-MB-231 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA) and was maintained in RPMI containing 10 % FBS, 1 % penicillin (10,000 units/ml), and 1 % streptomycin (10,000 μg/ml) (Hyclone Laboratories, Logan, UT, USA). Chemotherapy drugs (docetaxel, paclitaxel, carboplatin, cisplatin, vincristine, etoposide, irinotecan, doxorubicin) were acquired from the pharmacy at Health Sciences North, Sudbury, Ontario, Canada.