Author: Narendrula, Rashmi; Mispel-Beyer, Kyle; Guo, Baoqing; Parissenti, Amadeo M.; Pritzker, Laura B.; Pritzker, Ken; Masilamani, Twinkle; Wang, Xiaohui; Lannér, Carita
Title: RNA disruption is associated with response to multiple classes of chemotherapy drugs in tumor cell lines Document date: 2016_2_24
ID: 0mjizsoo_14
Snippet: Cells were harvested by scraping the adherent cells in lysis buffer and collecting them, along with any floating cells by centrifugation. Total RNA was isolated from harvested cells using miRNeasy kits (Qiagen Inc., Toronto, ON, CA). The quantity and integrity of isolated RNA was determined by capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies Canada, Inc., Mississauga, ON, CA) with known reference RNA standards of var.....
Document: Cells were harvested by scraping the adherent cells in lysis buffer and collecting them, along with any floating cells by centrifugation. Total RNA was isolated from harvested cells using miRNeasy kits (Qiagen Inc., Toronto, ON, CA). The quantity and integrity of isolated RNA was determined by capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies Canada, Inc., Mississauga, ON, CA) with known reference RNA standards of various masses. The RNA Disruption Index (RDI) was calculated for each sample using a proprietary algorithm (RNA Diagnostics, Inc., Toronto, ON, CA), which computes the ratio of abnormal to normal rRNAs [20] .
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