Author: Stagegaard, Julia; Kurth, Andreas; Stern, Daniel; Dabrowski, Piotr Wojciech; Pocknell, Ann; Nitsche, Andreas; Schrick, Livia
Title: Seasonal recurrence of cowpox virus outbreaks in captive cheetahs (Acinonyx jubatus) Document date: 2017_11_9
ID: 0oa4x05s_12
Snippet: Clinical specimens were homogenized in phosphate-buffered saline and added at different dilutions to confluent layers of Vero cells (ECACC, Cat. No 85020206) cultivated with media with 2% fetal calf serum supplemented with penicillin/streptomycin in 24-well cell culture plates. Once cytopathic effects were evident under the microscope, passaging was initiated......
Document: Clinical specimens were homogenized in phosphate-buffered saline and added at different dilutions to confluent layers of Vero cells (ECACC, Cat. No 85020206) cultivated with media with 2% fetal calf serum supplemented with penicillin/streptomycin in 24-well cell culture plates. Once cytopathic effects were evident under the microscope, passaging was initiated.
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