Selected article for: "cell lysate and infected cell"
Author: Stagegaard, Julia; Kurth, Andreas; Stern, Daniel; Dabrowski, Piotr Wojciech; Pocknell, Ann; Nitsche, Andreas; Schrick, Livia
Title: Seasonal recurrence of cowpox virus outbreaks in captive cheetahs (Acinonyx jubatus)
  • Document date: 2017_11_9
    • ID: 0oa4x05s_14
    Snippet: Serum samples were tested for antibodies by immunofluorescent assay (IFA) by using CPXVinfected HEp-2 cells and a FITC-conjugated goat-anti-human IgG (H+L) as described previously [24] or by indirect ELISA by using vaccinia virus-infected HEp-2 cell lysate as antigen. To this aim, UV-inactivated cell lysate was prepared by infecting HEp-2 cells (ATCC, CCL-23â„¢) with vaccinia virus strain New York City Board of Health (ATCC, VR-1536â„¢) by using .....
    Document: Serum samples were tested for antibodies by immunofluorescent assay (IFA) by using CPXVinfected HEp-2 cells and a FITC-conjugated goat-anti-human IgG (H+L) as described previously [24] or by indirect ELISA by using vaccinia virus-infected HEp-2 cell lysate as antigen. To this aim, UV-inactivated cell lysate was prepared by infecting HEp-2 cells (ATCC, CCL-23â„¢) with vaccinia virus strain New York City Board of Health (ATCC, VR-1536â„¢) by using RIPA buffer supplemented with protease inhibitor cocktail (Thermo Fisher Scientific) as described before [25] .

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