Author: Boey, Kenneth; Shiokawa, Kanae; Avsaroglu, Harutyun; Rajeev, Sreekumari
Title: Seroprevalence of Rodent Pathogens in Wild Rats from the Island of St. Kitts, West Indies Document date: 2019_5_10
ID: 000q5l5n_8
Snippet: Captured rats were euthanized using carbon dioxide gas (CO 2 ) and cervical dislocation. This was performed by placing traps containing the rats in a leak-proof plastic bag and filling it with CO 2 . Once the rats were visibly unconscious, they were taken out of the plastic bag and cervical dislocation was performed as secondary euthanasia. Subsequently, blood was collected by open cardiac puncture. We recorded the weight, sex, and body length of.....
Document: Captured rats were euthanized using carbon dioxide gas (CO 2 ) and cervical dislocation. This was performed by placing traps containing the rats in a leak-proof plastic bag and filling it with CO 2 . Once the rats were visibly unconscious, they were taken out of the plastic bag and cervical dislocation was performed as secondary euthanasia. Subsequently, blood was collected by open cardiac puncture. We recorded the weight, sex, and body length of each rat. Serum was obtained after centrifugation of whole blood, and stored at −80 • C until used for serological analysis. The rat species was determined by amplification and sequencing of the mitochondrial cytochrome b gene [5] . Sequences were aligned by Molecular Evolutionary Genetic Analysis version 7.0 (MEGA7) [6] and a search of homologous sequences was performed using Basic Local Alignment Search Tool (BLAST) [7] .
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