Selected article for: "conventional PCR and real time"
Author: Zhang, Chi; Xiu, Leshan; Xiao, Yan; Xie, Zhengde; Ren, Lili; Peng, Junping
Title: Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry
  • Document date: 2018_4_5
    • ID: 0k2blp7n_15
    Snippet: Real-time PCR was performed to confirm positive results from the BP-MS method. For S. pneumoniae, H. influenzae, S. aureus, M. catarrhalis, K. pneumoniae, A. baumannii, and P. aeruginosa, multiplex real-time PCR was used (Gadsby et al., 2015) . For N. meningitidis, L. pneumophila, M. pneumoniae, and B. pertussis, singleplex real-time PCR was used according to previously published methods (Corless et al., 2001; Welti et al., 2003; Tatti et al., 20.....
    Document: Real-time PCR was performed to confirm positive results from the BP-MS method. For S. pneumoniae, H. influenzae, S. aureus, M. catarrhalis, K. pneumoniae, A. baumannii, and P. aeruginosa, multiplex real-time PCR was used (Gadsby et al., 2015) . For N. meningitidis, L. pneumophila, M. pneumoniae, and B. pertussis, singleplex real-time PCR was used according to previously published methods (Corless et al., 2001; Welti et al., 2003; Tatti et al., 2011) . Each sample was tested in duplicate and regarded as positive if both cycle threshold (CT) values were <40. Nested PCR methods were used to resolve disagreements between results of the BP-MS and realtime PCR methods. The nested PCR was designed in-house for this study and was based on published conventional PCR assays. The primers and probes used in the confirmatory tests described above are listed in Tables S2, S3 in the Supplementary Material.

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