Author: Laine, Romain F.; Albecka, Anna; van de Linde, Sebastian; Rees, Eric J.; Crump, Colin M.; Kaminski, Clemens F.
Title: Structural analysis of herpes simplex virus by optical super-resolution imaging Document date: 2015_1_22
ID: 0zchxz00_7
Snippet: Multi-colour dSTORM resolves protein layers in HSV-1. We used two-colour dSTORM imaging of purified virions to investigate the architecture of different protein layers within individual virus particles. The use of Alexa Fluor (AF) 647 is well established in localization microscopy, owing to its favourable photoswitching behaviour and its high photostability and quantum yield. We thus searched for a second fluorophore that could be reliably used i.....
Document: Multi-colour dSTORM resolves protein layers in HSV-1. We used two-colour dSTORM imaging of purified virions to investigate the architecture of different protein layers within individual virus particles. The use of Alexa Fluor (AF) 647 is well established in localization microscopy, owing to its favourable photoswitching behaviour and its high photostability and quantum yield. We thus searched for a second fluorophore that could be reliably used in combination with AF647 for imaging virus particles and tested AF647/ATTO532, AF647/AF546 and AF647/AF568 in a range of photoswitching environments. All fluorophores were linked to secondary antibodies and imaging protocols were optimized for each fluorophore combination (Fig. 1a) .
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