Author: Boyington, Jeffrey C.; Joyce, M. Gordon; Sastry, Mallika; Stewart-Jones, Guillaume B. E.; Chen, Man; Kong, Wing-Pui; Ngwuta, Joan O.; Thomas, Paul V.; Tsybovsky, Yaroslav; Yang, Yongping; Zhang, Baoshan; Chen, Lei; Druz, Aliaksandr; Georgiev, Ivelin S.; Ko, Kiyoon; Zhou, Tongqing; Mascola, John R.; Graham, Barney S.; Kwong, Peter D.
Title: Structure-Based Design of Head-Only Fusion Glycoprotein Immunogens for Respiratory Syncytial Virus Document date: 2016_7_27
ID: 1nbocmux_45
Snippet: The four top scoring head-only immunogens were expressed in one liter cultures of Expi293F cells. After affinity purification and cleavage of purification tags, size exclusion chromatography was used to characterize their oligomeric states, and appropriate peaks pooled for further characterization. Design i-273 eluted as three peaks. Because it was designed as a monomer we chose the peak corresponding to the monomer for further characterization. .....
Document: The four top scoring head-only immunogens were expressed in one liter cultures of Expi293F cells. After affinity purification and cleavage of purification tags, size exclusion chromatography was used to characterize their oligomeric states, and appropriate peaks pooled for further characterization. Design i-273 eluted as three peaks. Because it was designed as a monomer we chose the peak corresponding to the monomer for further characterization. Designs i-693, i-210, and i-447 eluted primarily as single peaks, corresponding to dimer, trimer and trimer, respectively (Fig 2) .
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