Author: Fan, Qing; Kopp, Sarah J.; Connolly, Sarah A.; Longnecker, Richard
Title: Structure-Based Mutations in the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Arm Impart a Slow-Entry Phenotype Document date: 2017_5_16
ID: 1v6nf28a_18
Snippet: By using a low pH to inactivate extracellular virus at defined time points, we demonstrated that the gB 3A viruses have remarkably delayed penetration of cells. Penetration of Vero cells by WT HSV-1 was detectable after 20 min at 37°C and reached a maximum after 2 h at 37°C (Fig. 5A) . In contrast, the gB 3A viruses did not penetrate the cells until 10 to 12 h at 37°C (Fig. 5C and D) . HSV entry into Vero cells occurs at the cell surface (36, .....
Document: By using a low pH to inactivate extracellular virus at defined time points, we demonstrated that the gB 3A viruses have remarkably delayed penetration of cells. Penetration of Vero cells by WT HSV-1 was detectable after 20 min at 37°C and reached a maximum after 2 h at 37°C (Fig. 5A) . In contrast, the gB 3A viruses did not penetrate the cells until 10 to 12 h at 37°C (Fig. 5C and D) . HSV entry into Vero cells occurs at the cell surface (36, 37) , and thus, the delayed penetration kinetics of gB 3A may be due to a defect in the ability of gB 3A to mediate fusion, rather than an effect on endocytosis.
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