Author: Wang, Qing S.; Jan, Eric
Title: Switch from Cap- to Factorless IRES-Dependent 0 and +1 Frame Translation during Cellular Stress and Dicistrovirus Infection Document date: 2014_8_4
ID: 0if5z3xp_11
Snippet: Unless indicated, cells were harvested 6 hour after transfection by centrifugation at 9600 g for 1 minute, followed by wash with 1 ml of PBS. The cells pellets were then resuspended in 40 ml of Passive Lysis Buffer (Promega) and stored at -20uC. The concentration of protein in each lysate was determined by Bradford assay......
Document: Unless indicated, cells were harvested 6 hour after transfection by centrifugation at 9600 g for 1 minute, followed by wash with 1 ml of PBS. The cells pellets were then resuspended in 40 ml of Passive Lysis Buffer (Promega) and stored at -20uC. The concentration of protein in each lysate was determined by Bradford assay.
Search related documents:
Co phrase search for related documents- cell pellet and protein concentration: 1, 2, 3, 4
- lysate protein concentration and protein concentration: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date