Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p Document date: 2014_8_13
ID: 1i6hni9s_11
Snippet: For condition medium collection, various numbers of OFSCs (OFSC/macrophage ratio from 0.5 to 4) were plated in six-well culture plates. The condition medium was collected from OFSCs under 24-hour starvation. Macrophages were then treated with 1:1 mixed medium (one-half DMEM/Ham's F12 medium for macrophages and one-half OFSCs-CM). After 6 hours of OFSC-CM treatment, studies on functional analysis of macrophages were performed......
Document: For condition medium collection, various numbers of OFSCs (OFSC/macrophage ratio from 0.5 to 4) were plated in six-well culture plates. The condition medium was collected from OFSCs under 24-hour starvation. Macrophages were then treated with 1:1 mixed medium (one-half DMEM/Ham's F12 medium for macrophages and one-half OFSCs-CM). After 6 hours of OFSC-CM treatment, studies on functional analysis of macrophages were performed.
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