Selected article for: "culture plate and tissue culture"
Author: Andrabi, Raiees; Pallesen, Jesper; Allen, Joel D.; Song, Ge; Zhang, Jinsong; de Val, Natalia; Gegg, Gavin; Porter, Katelyn; Su, Ching-Yao; Pauthner, Matthias; Newman, Amanda; Bouton-Verville, Hilary; Garces, Fernando; Wilson, Ian A.; Crispin, Max; Hahn, Beatrice H.; Haynes, Barton F.; Verkoczy, Laurent; Ward, Andrew B.; Burton, Dennis R.
Title: The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template
  • Document date: 2019_5_21
    • ID: 1ni7949q_48
    Snippet: Neutralization was measured by using single-round replication-defective HIV Env-pseudoviruses and TZM-bl target cells (Montefiori, 2005; Seaman et al., 2010) . 25ul of 3-fold serially diluted mAbs or serum samples were pre-incubated at 37 C for 1h with 25ul of tissue culture infective dose-50 (TCID50) Env-pseudotyped virus in a half-area 96-well tissue culture plate. TZM-bl target cells (5,000 cells/well) in 50ml of DMEM were added and the plates.....
    Document: Neutralization was measured by using single-round replication-defective HIV Env-pseudoviruses and TZM-bl target cells (Montefiori, 2005; Seaman et al., 2010) . 25ul of 3-fold serially diluted mAbs or serum samples were pre-incubated at 37 C for 1h with 25ul of tissue culture infective dose-50 (TCID50) Env-pseudotyped virus in a half-area 96-well tissue culture plate. TZM-bl target cells (5,000 cells/well) in 50ml of DMEM were added and the plates were allowed to grow in humidified incubator at 37 C and 5% Co 2 . The luciferase activity of the lysed cells was read on instrument (Biotek) after 2-3 days, by adding lysis buffer followed by Brightglow (Promega). The 50% inhibitory concentration (IC 50 ) or 50% inhibitory doses (ID 50 ) was reported as the antibody concentration or serum dilution required to reduce infection by half.

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