Author: Hoffmann, Markus; Krüger, Nadine; Zmora, Pawel; Wrensch, Florian; Herrler, Georg; Pöhlmann, Stefan
Title: The Hemagglutinin of Bat-Associated Influenza Viruses Is Activated by TMPRSS2 for pH-Dependent Entry into Bat but Not Human Cells Document date: 2016_3_30
ID: 0ejhd9nw_26
Snippet: Endosomal low pH triggers FLUAV-HA for membrane fusion. Therefore, we investigated whether increasing the endosomal pH in EpoNi/22.1 cells by ammonium chloride treatment impacts HAL-driven entry. As expected, ammonium chloride treatment led to a decrease in transduction efficiency mediated by pseudotypes bearing the HA-proteins of FLUAV of the H1N1 and H2N2 subtype and VSV-G (Fig 5) . In contrast, pseudotype entry orchestrated by NiV-F and -G was.....
Document: Endosomal low pH triggers FLUAV-HA for membrane fusion. Therefore, we investigated whether increasing the endosomal pH in EpoNi/22.1 cells by ammonium chloride treatment impacts HAL-driven entry. As expected, ammonium chloride treatment led to a decrease in transduction efficiency mediated by pseudotypes bearing the HA-proteins of FLUAV of the H1N1 and H2N2 subtype and VSV-G (Fig 5) . In contrast, pseudotype entry orchestrated by NiV-F and -G was unaffected, again in keeping with published data [52, 53] . Finally, HALdriven entry was markedly reduced by ammonium chloride, demonstrating that the membrane fusion activity of batFLUAV-HAL is triggered by acidification (Fig 5) .
Search related documents:
Co phrase search for related documents- ammonium chloride and fusion activity: 1, 2, 3, 4
- ammonium chloride and low pH trigger: 1
- ammonium chloride and membrane fusion: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- ammonium chloride and pH trigger: 1
- ammonium chloride and pseudotype entry: 1
- ammonium chloride treatment and chloride treatment: 1, 2, 3, 4, 5, 6, 7
- ammonium chloride treatment and fusion activity: 1
- ammonium chloride treatment and low pH trigger: 1
- ammonium chloride treatment and membrane fusion: 1, 2, 3
- ammonium chloride treatment and pH trigger: 1
- chloride treatment and fusion activity: 1
- chloride treatment and low pH trigger: 1
- chloride treatment and membrane fusion: 1, 2, 3
- chloride treatment and pH trigger: 1
- endosomal increase and fusion activity: 1
- endosomal increase and membrane fusion: 1
- fusion activity and markedly reduce: 1
- fusion activity and membrane fusion: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- fusion activity and pH trigger: 1
Co phrase search for related documents, hyperlinks ordered by date