Author: Guo, Huichen; Huang, Mei; Yuan, Quan; Wei, Yanquan; Gao, Yuan; Mao, Lejiao; Gu, Lingjun; Tan, Yong Wah; Zhong, Yanxin; Liu, Dingxiang; Sun, Shiqi
Title: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain Document date: 2017_1_12
ID: 0238bsxb_11
Snippet: Each sample was lysed in TNEV buffer containing a cocktail of protease and phosphatase inhibitors. Protein concentration was determined with Bio-Rad Protein Assay kit. Then, the lysate was added at the same volume as the 2× SDS sample loading buffer with bromophenol blue. Equal amounts of total protein were separated by SDS-PAGE and then transferred onto polyvinylidene difluoride membranes. The membranes were incubated with primary antibody for .....
Document: Each sample was lysed in TNEV buffer containing a cocktail of protease and phosphatase inhibitors. Protein concentration was determined with Bio-Rad Protein Assay kit. Then, the lysate was added at the same volume as the 2× SDS sample loading buffer with bromophenol blue. Equal amounts of total protein were separated by SDS-PAGE and then transferred onto polyvinylidene difluoride membranes. The membranes were incubated with primary antibody for 1 h at 37˚C, and then with HRP-conjugated secondary antibody for an additional 1 h at 37˚C. The membranes were detected with ECL Advance Western Blot Detection Kit (Amersham, Marlborough, MA, USA).
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