Selected article for: "cdna target and gene primer"
Author: Guo, Huichen; Huang, Mei; Yuan, Quan; Wei, Yanquan; Gao, Yuan; Mao, Lejiao; Gu, Lingjun; Tan, Yong Wah; Zhong, Yanxin; Liu, Dingxiang; Sun, Shiqi
Title: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain
  • Document date: 2017_1_12
    • ID: 0238bsxb_15
    Snippet: Vero cells were seeded on 35-mm dishes and infected with 1 MOI IBV when the cell monolayer was approximately 90% confluent. The infected cells were treated with MβCD or Mevastatin for 1 h at 4˚C and harvested at certain time points [0, 8, 12, 18 , and 24 h post-infection (hpi)]. Total RNA was extracted, and the cDNA template was synthesized with PrimeScript TM Reverse Transcriptase (Takara, Dalian, China). The target gene was amplified by PCR w.....
    Document: Vero cells were seeded on 35-mm dishes and infected with 1 MOI IBV when the cell monolayer was approximately 90% confluent. The infected cells were treated with MβCD or Mevastatin for 1 h at 4˚C and harvested at certain time points [0, 8, 12, 18 , and 24 h post-infection (hpi)]. Total RNA was extracted, and the cDNA template was synthesized with PrimeScript TM Reverse Transcriptase (Takara, Dalian, China). The target gene was amplified by PCR with synthesized cDNA and specific primer pairs. The primers used for amplification are listed in Table 1 . The amplification program was set at 94˚C for 25 s, 56˚C for 25 s, and 72˚C for 20 s for 20 cycles. The sizes and specificity of the PCR products were verified by agarose gel electrophoresis. The relative expression levels were calculated by the 2 -ΔΔCT method against the uninfected controls. GADPH gene was set as the endogenous control.

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