The PD-1/PD-L1 Pathway Affects the Expansion and Function of Cytotoxic CD8(+) T Cells During an Acute Retroviral Infection

David, Paul; Megger, Dominik A.; Kaiser, Tamara; Werner, Tanja; Liu, Jia; Chen, Lieping; Sitek, Barbara; Dittmer, Ulf; Zelinskyy, Gennadiy

FVD positive cells were excluded from the analyses. Intracellular granzyme A (GzA-3G8.5), granzyme B (GB11, Invitrogen, Darmstadt, Germany), granzyme K (orb102688), activated caspase 3, and Ki67 stainings were performed as described (14) . Data were acquired on a LSR II flow cytometer (Becton Dickinson) from 200,000 to 300,000 lymphocyte-gated events per sample. Analyses were done using FlowJo (Treestar) and FACSDiva software (Becton Dickinson). The quantity of survivor cells at day 10 was calculated by determining the portion of cells detectible at day 10 after infection from the numbers of infected cells at the peak of FV infection at day 6. These quantities were calculated for every cell population separately.