Author: Tan, Jinzhi; Vonrhein, Clemens; Smart, Oliver S.; Bricogne, Gerard; Bollati, Michela; Kusov, Yuri; Hansen, Guido; Mesters, Jeroen R.; Schmidt, Christian L.; Hilgenfeld, Rolf
Title: The SARS-Unique Domain (SUD) of SARS Coronavirus Contains Two Macrodomains That Bind G-Quadruplexes Document date: 2009_5_15
ID: 1aqt65cc_28
Snippet: For SUD-M, a recent publication [29] reported binding to oligo(A). However, we fail to observe this ( Figure 4A, lane labeled ''A'') . Instead, we have demonstrated that full-length SUD and SUD core bind oligodeoxynucleotides and oligoribonucleotides that form G-quadruplexes. For full-length SUD and SUD core , we had previously shown binding to oligo(dG) and oligo(G) stretches [31] , but the demonstration here of oligo(dG) binding to the individ.....
Document: For SUD-M, a recent publication [29] reported binding to oligo(A). However, we fail to observe this ( Figure 4A, lane labeled ''A'') . Instead, we have demonstrated that full-length SUD and SUD core bind oligodeoxynucleotides and oligoribonucleotides that form G-quadruplexes. For full-length SUD and SUD core , we had previously shown binding to oligo(dG) and oligo(G) stretches [31] , but the demonstration here of oligo(dG) binding to the individual SUD core subdomains, SUD-N and SUD-M, is unexpected because their overall electrostatic properties are very different from one another: SUD-N is acidic (pI = 5.3), whereas SUD-M is basic (pI = 9.0). However, even SUD-N shows surface patches with positive electrostatics that could bind nucleic acid ( Figure 3B) .
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