Author: Jemielity, Stephanie; Wang, Jinyize J.; Chan, Ying Kai; Ahmed, Asim A.; Li, Wenhui; Monahan, Sheena; Bu, Xia; Farzan, Michael; Freeman, Gordon J.; Umetsu, Dale T.; DeKruyff, Rosemarie H.; Choe, Hyeryun
Title: TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine Document date: 2013_3_28
ID: 0fais1pz_7
Snippet: HEK293T (human embryonic kidney), Huh7 (human hepatocarcinoma), 3T3 (murine embryonic fibroblast) and MDCK (canine kidney) cells were grown at 37uC in DMEM supplemented with 10% fetal bovine serum (FBS), 1 mM sodium pyruvate and 1% penicillin/streptomycin (P/S). NPC1 2/2 and NPC1 2/2 mNPC1 CHO cells (also referred to as M12 and wt8, respectively [36] ) were kept at 37uC in DMEM/F12 with 5% FBS and 1% P/S. These cells were gifts from Daniel S. Ory.....
Document: HEK293T (human embryonic kidney), Huh7 (human hepatocarcinoma), 3T3 (murine embryonic fibroblast) and MDCK (canine kidney) cells were grown at 37uC in DMEM supplemented with 10% fetal bovine serum (FBS), 1 mM sodium pyruvate and 1% penicillin/streptomycin (P/S). NPC1 2/2 and NPC1 2/2 mNPC1 CHO cells (also referred to as M12 and wt8, respectively [36] ) were kept at 37uC in DMEM/F12 with 5% FBS and 1% P/S. These cells were gifts from Daniel S. Ory at Washington University School of Medicine. C6/36 Aedes albopictus mosquito cells were maintained at 28uC in DMEM containing 10% FBS, 1 mM sodium pyruvate and 1% P/S.
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