Selected article for: "human exposure and post exposure"
Author: Zapata, Juan Carlos; Carrion, Ricardo; Patterson, Jean L.; Crasta, Oswald; Zhang, Yan; Mani, Sachin; Jett, Marti; Poonia, Bhawna; Djavani, Mahmoud; White, David M.; Lukashevich, Igor S.; Salvato, Maria S.
Title: Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate
  • Document date: 2013_9_12
    • ID: 0epeljaf_13
    Snippet: Human PBMC and DC exposure 1.2610 7 PBMC from healthy donors were exposed to 1 MOI of LASV or ML29 (BSL-4 facilities at SFBR, San Antonio, TX) for 45 minutes at 37uC in 5% CO 2 and plated in 6 well plates in duplicate. Supernatants and cells were harvested at 4, 8, and 24 hours post-exposure. Cells were re-suspended in Trizol and kept at 270uC for RNA isolation. 1610 7 DC from 3 healthy donors were exposed to 1 MOI of LASV, ML29 or LPS (as an inh.....
    Document: Human PBMC and DC exposure 1.2610 7 PBMC from healthy donors were exposed to 1 MOI of LASV or ML29 (BSL-4 facilities at SFBR, San Antonio, TX) for 45 minutes at 37uC in 5% CO 2 and plated in 6 well plates in duplicate. Supernatants and cells were harvested at 4, 8, and 24 hours post-exposure. Cells were re-suspended in Trizol and kept at 270uC for RNA isolation. 1610 7 DC from 3 healthy donors were exposed to 1 MOI of LASV, ML29 or LPS (as an inhibitor control: LPS is usually a positive control, here it was used to inhibit thrombomodulin) for 45 minutes at 37uC in 5% CO 2 (BSL-4 facilities at CDC, Atlanta, GA). Supernatants and cells were harvested at 24 hours post-exposure. Supernatants were kept at 270uC for ELISA analysis.

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