Author: Zapata, Juan Carlos; Carrion, Ricardo; Patterson, Jean L.; Crasta, Oswald; Zhang, Yan; Mani, Sachin; Jett, Marti; Poonia, Bhawna; Djavani, Mahmoud; White, David M.; Lukashevich, Igor S.; Salvato, Maria S.
Title: Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate Document date: 2013_9_12
ID: 0epeljaf_37
Snippet: Exposure of human PBMC to LASV resulted in over-expression of all 17 IFN-stimulated genes by 2-to 100-fold over background. Exposure of PBMC to ML29 had relatively little effect on the 17 tested genes. Comparative analyses showed a good correlation (76%) between the two techniques, DNA array and IFN qRT-PCR array (Table 1) . For a few genes measured in the DNA array, ML29 exposure gave a higher background than quantitation by real-time PCR. This .....
Document: Exposure of human PBMC to LASV resulted in over-expression of all 17 IFN-stimulated genes by 2-to 100-fold over background. Exposure of PBMC to ML29 had relatively little effect on the 17 tested genes. Comparative analyses showed a good correlation (76%) between the two techniques, DNA array and IFN qRT-PCR array (Table 1) . For a few genes measured in the DNA array, ML29 exposure gave a higher background than quantitation by real-time PCR. This is because the housekeeping genes we used for normalizing the PCR data, including GAPDH, were not constantly expressed in the compared data sets, whereas probesets used for normalizing the microarray data varied less than 0.04% in all data sets (see Methods).
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