Biological Evaluation of Molecules of the azaBINOL Class as Antiviral Agents: Specific Inhibition of HIV-1 RNase H Activity by 7-Isopropoxy-8-(naphth-1-yl)quinoline

Ross D. Overacker; Somdev Banerjee; George F. Neuhaus; Selena Milicevic Sephton; Alexander Herrmann; James A. Strother; Ruth Brack-Werner; Paul R. Blakemore; Sandra Loesgen

Testing for complexation of B#24 with Mg 2+ ions was carried out following previous 548 protocols with adjustments. 50 In brief, a 1 M stock solution of MgCl2 and a 1 M solution of B#24 549 were prepared in 1:1 ethanol/acetonitrile mixtures. B#24 was diluted to 100 µM and UV 550 absorbance readings recorded using a BioTek synergy plate reader from 200 -400 nm. 10 µL 551 additions of a 500 mM Mg 2+ solution containing 100 µM B#24 (to keep compound concentration 552 consistent) was added stepwise followed by absorbance readings between each addition. The 553 concentration of Mg 2+ was raised with each addition from 0.5 mM to 120 mM. Alignment, 554 reference subtraction of blanks, and plotting were done on raw data using Microsoft Excel. 555