Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector

Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.

These results demonstrated that all three RV structural proteins VP2, VP6, and VP7 were synthesized from the newly designed HSV-1 cassettes in human and non-human cells and the codon-optimization led to an increased quantity of generated RV proteins in human cells. Next, the subcellular distribution of the different RV structural proteins in HSV-1 vector transduced Vero2-2 cells was examined by confocal laser scanning microscopy and specific antibodies ( Figure 1D ). The VP2 signal was detected as punctate pattern distributed throughout the cytoplasm and VP6 expression appeared as discrete punctate and circular structures in the cytoplasm. V5-tagged VP7 was detected in the cytoplasm as dot structures, some forming aggregates. When compared to the concanavalin A (ConA) staining of the endoplasmic reticulum (ER) membranes, VP7 was localized at the ER as previously described [15, 21] .