Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector

Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.

In a next step, HepG2 cells were transduced with sWa[VP2/6/7] and ultrathin sections were prepared for transmission electron microscopy (TEM) (Figure 2A,B) . The structures detected resembled RV particles or viroplasms [22] . To prove that these structures represented accumulated RV proteins, correlative light and electron microscopy [23] was done ( Figure 2C ,D). The ultrathin sections of vector-transduced cells were labeled as indicated in the Materials and Methods section and imaged with a fluorescence microscope. The resulting micrograph was used as a map to record the same area in the electron microscope (EM). Figure 2C shows an overlay of the fluorescent and the EM images and Figure 2D is an enlargement of the area of interest recorded by the EM, focusing on the highly RV-antibody-positive marked and electron-dense regions. Such electron-dense and antibody-positive regions showed regularly shaped circular structures that formed aggregates or clusters resembling virus particles.