Selected article for: "luc reporter and luciferase reporter"
Author: Qian, Shaoju; Gao, Zitong; Cao, Rui; Yang, Kang; Cui, Yijie; Li, Shaowen; Meng, Xianrong; He, Qigai; Li, Zili
Title: Transmissible Gastroenteritis Virus Infection Up-Regulates FcRn Expression via Nucleocapsid Protein and Secretion of TGF-ß in Porcine Intestinal Epithelial Cells
  • Document date: 2020_1_21
    • ID: 06qddkw0_7
    Snippet: Luciferase reporter plasmids FcRn-Luc, NF-κB-Luc, pR-TK, p65-EGFP, and p65-Tag2B were prepared in our laboratory and have been described previously. Genes encoding TGEV proteins were amplified from the genomic RNA of the TGEV strain WH-1 and then cloned into the expression vector pCAGGS-HA (Guo et al., 2016b) (Table 1) . pCAGGS-N was used as a template to amplify several deletion mutants of the virus N gene. These mutants were then cloned into t.....
    Document: Luciferase reporter plasmids FcRn-Luc, NF-κB-Luc, pR-TK, p65-EGFP, and p65-Tag2B were prepared in our laboratory and have been described previously. Genes encoding TGEV proteins were amplified from the genomic RNA of the TGEV strain WH-1 and then cloned into the expression vector pCAGGS-HA (Guo et al., 2016b) (Table 1) . pCAGGS-N was used as a template to amplify several deletion mutants of the virus N gene. These mutants were then cloned into the pCAGGS-HA ( Table 2) . Small interfering RNA (siRNA) molecules targeting TLR2, TLR3, TLR4, TLR8, TLR9, RIG-I, MyD88, TRIF, and negative controls were obtained from Shanghai GenePharma ( Table 3) . MAPK inhibitors SB203580, SP600125, U0126 and DMSO were purchased from Sigma-Aldrich (United States).

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