Author: Qian, Shaoju; Gao, Zitong; Cao, Rui; Yang, Kang; Cui, Yijie; Li, Shaowen; Meng, Xianrong; He, Qigai; Li, Zili
Title: Transmissible Gastroenteritis Virus Infection Up-Regulates FcRn Expression via Nucleocapsid Protein and Secretion of TGF-ß in Porcine Intestinal Epithelial Cells Document date: 2020_1_21
ID: 06qddkw0_9
Snippet: Total RNA was prepared using TRIzol @ reagent (Invitrogen). RT-qPCR was performed using SYBR Green PCR Master Mix (Takara, Osaka, Japan) in the Bio-Rad Sequence Detection System (Bio-Rad). Each transcript from each sample was determined three times. The delta threshold cycle (2 − CT ) method was used to calculate the fold-change compared to the normal gene expression. The specific pig gene primers ( Table 4) were designed using Primer Express s.....
Document: Total RNA was prepared using TRIzol @ reagent (Invitrogen). RT-qPCR was performed using SYBR Green PCR Master Mix (Takara, Osaka, Japan) in the Bio-Rad Sequence Detection System (Bio-Rad). Each transcript from each sample was determined three times. The delta threshold cycle (2 − CT ) method was used to calculate the fold-change compared to the normal gene expression. The specific pig gene primers ( Table 4) were designed using Primer Express software (version 3.0; Applied Biological Systems, Carlsbad, CA, United States) as previously described with some modifications (Temeeyasen et al., 2018) .
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