Two Novel Parvoviruses in Frugivorous New and Old World Bats

Canuti, Marta; Eis-Huebinger, Anna Maria; Deijs, Martin; de Vries, Michel; Drexler, Jan Felix; Oppong, Samuel K.; Müller, Marcel A.; Klose, Stefan M.; Wellinghausen, Nele; Cottontail, Veronika M.; Kalko, Elisabeth K. V.; Drosten, Christian; van der Hoek, Lia

To identify conserved parts between Parvovirinae a Simplot analysis was performed. In the first putative ORF (nucleotides 84-1961 of Aj-BtPV-1, coding for a putative non structural protein NS1 of 625 aa) it was possible to identify a more conserved region of approx. 500 nucleotides. This region (nucleotides 835-1320 of Aj-BtPV-1 NS1 ORF) was then used to perform additional alignments and create further phylogenetic trees (both at nucleotide and amino acid level). The top identity percentages of Aj-BtPV-1 were found with Dependoviruses (between 53.4 and 59.6% at the amino acid level). However, all trees were consistent with the previous ones and Aj-BtPV-1 was still forming a separate lineage (branching autonomously from the others) confirming its independence from all the other known genera.