UNC93B1 Mediates Innate Inflammation and Antiviral Defense in the Liver during Acute Murine Cytomegalovirus Infection

Crane, Meredith J.; Gaddi, Pamela J.; Salazar-Mather, Thais P.

Portions of the median liver lobes were isolated, fixed in 10% neutral buffered formalin, and paraffin embedded. Tissue sections (5 mm) were stained with H&E and analyzed microscopically. Images shown were photographed at the indicated magnification with a DP70 digital camera and software (Optical Analysis Corporation). Inflammatory foci, defined as discrete clusters containing between 6-60 nucleated cells, were quantitated as described previously [23] . In brief, inflammatory foci were identified, at a magnification of 200, as clusters of cells in totals of 8650 mm 2 areas per representative tissue. In some cases, liver sections had larger areas of inflammation with .60 cells with less defined foci, and are indicated as such. Numbers of nucleated cells per inflammatory foci were counted in 20 individual foci per representative tissue at a magnification of 400. Liver alanine aminotransferase (ALT) was measured in serum samples by Marshfield Labs (Marshfield, WI).