Author: Romagosa, Anna; Allerson, Matt; Gramer, Marie; Joo, Han Soo; Deen, John; Detmer, Susan; Torremorell, Montserrat
Title: Vaccination of influenza a virus decreases transmission rates in pigs Document date: 2011_12_20
ID: 0q8fedqf_24
Snippet: Virus isolation and titration was performed only from the nasal swabs collected from the seeder pigs prior to commingling, and from tissues at necropsy on MDCK cell monolayers [37] . Supernatants showing cytopathic effect (CPE) were titrated using ten-fold serial dilution and expressed as a log 10 TCID 50 /mL calculated by the Spearman-Kärber method [39] ......
Document: Virus isolation and titration was performed only from the nasal swabs collected from the seeder pigs prior to commingling, and from tissues at necropsy on MDCK cell monolayers [37] . Supernatants showing cytopathic effect (CPE) were titrated using ten-fold serial dilution and expressed as a log 10 TCID 50 /mL calculated by the Spearman-Kärber method [39] .
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