Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses

He, Biao; Li, Zuosheng; Yang, Fanli; Zheng, Junfeng; Feng, Ye; Guo, Huancheng; Li, Yingying; Wang, Yiyin; Su, Nan; Zhang, Fuqiang; Fan, Quanshui; Tu, Changchun

The family Iflaviridae has a single genus Iflavirus consisting of 7 viral species [48] . By Solexa sequencing, 191 and 286 contigs annotated to Iflaviridae were found in groups XM and WM respectively, showing high identity with Ectropis obliqua virus (EOV) and Perina nuda virus (PNV), both being insect viral species within genus Iflavirus (Table 3) . Nested RT-PCR of all bat organs targeting 369 nt at the 39 end of the single ORF confirmed the results of Solexa sequencing, and further showed that 18% (56/ 320) of guts of M. fuliginosus in WM and 8% (26/320) of M. fuliginosus in XM were positive for the genus Iflavirus. Sequences (Accession numbers: JX863712-JX863717) amplified from the two groups had .98% nt identity, indicating that the viruses from the two groups were the same variant, sharing 82% aa identity with PNV and 80% identity with EOV ( Figure 2 ) [49] .